Scientific Article
previous document  unige:11638  next document
add to browser collection

A chimeric cell adhesion molecule mediates homing of lymphocytes to vascularized tumors

Published in Cancer Research. 1999, vol. 59, no. 20, p. 5255-5263
Abstract To facilitate tumor colonization by adoptively transferred cells of the immune system, we created a chimeric cell adhesion molecule that mediates tumor-specific homing by binding to the integrin alpha(v)beta3 on angiogenic endothelial cells. A high-affinity cell adhesion molecule for integrin alpha(v)beta3 was generated by fusing the disintegrin kistrin to the transmembrane adhesion molecule CD31/PECAM-1. This chimeric cell adhesion molecule, termed KISS31, mediates adhesion of lymphoid cells to soluble recombinant integrin alpha(v)beta3 and to endotheliomal monolayers in vitro. KISS31-expressing lymphoid cells accumulate in angiogenic tumors in two in vivo models, in B16/129 melanoma xenografts on the chick chorioallantois and in s.c. growing Lewis lung carcinoma in mice. Our data indicate that expression of KISS31 on lymphoid cells confers tumor-specific homing. This is, to our knowledge, the first example of an experimental mechanism that targets living cells to tumors by redirecting their homing pattern.
Keywords Amino Acid SequenceAnimalsAntigens, CD31/ physiologyBase SequenceCell MovementChickensImmunotherapy, AdoptiveLymphocytes/ immunology/physiologyMiceMice, Inbred C57BLMolecular Sequence DataNeoplasms, Experimental/ therapyPeptides/ physiologyReceptors, Vitronectin/physiologyRecombinant Fusion Proteins/ physiologyTumor Cells, Cultured
Stable URL
Full text
PMID: 10537306
134 hits and 1 download since 2010-08-27
Export document
Format :
Citation style :