Scientific Article
previous document  unige:11492  next document
add to browser collection

Glycoprotein IIb-IIIa is expressed on avian multilineage hematopoietic progenitor cells

Vaigot, P.
Quere, P.
Corbel, C.
Published in Blood. 1999, vol. 93, no. 9, p. 2898-2906
Abstract The fibrinogen receptor GPIIb-IIIa integrin is known to be expressed on cells of the megakaryocytic lineage, but its presence on hematopoietic progenitors has been a controversial issue. To resolve this ambiguity unequivocally, we performed clonogenic assays and intrathymic cell-transfer experiments in congenic animals. As the ontogeny of the avian hematopoietic system is well documented, we used this experimental model to trace GPIIb-IIIa expression during embryogenesis. Consequently, we now report that the GPIIb-IIIa integrin is expressed as early as embryonic day 3.5 (E3.5) to 4 in intraaortic hematopoietic clusters, the first site of intraembryonic hematopoietic progenitor emergence, and later in E6 paraaortic foci. Myeloid and erythroid progenitors were also detected within the GPIIb-IIIa+ CD45(+) population isolated from the E3.5 to 4 aortic area, while in embryonic and adult bone marrow, myeloid, erythroid, and T-cell progenitors were present in the GPIIb-IIIa+ c-kit+ population. Furthermore, we also provide the first evidence, that GPIIb-IIIa+ bone marrow cells can differentiate into T cells. Hence, GPIIb-IIIa can be used as a marker for multilineage hematopoietic progenitors, permitting identification of early intraembryonic sites of hematopoiesis, as well as the isolation of embryonic and adult hematopoietic progenitors.
Keywords AnimalsAorta/embryologyBlood Platelets/ cytology/physiologyBone Marrow/embryologyBone Marrow Cells/ cytology/physiologyCell Differentiation/drug effectsCells, CulturedChick EmbryoChickensColony-Forming Units AssayErythrocytes/cytology/physiologyGene Expression Regulation, DevelopmentalGranulocytes/cytology/physiologyHematopoietic Stem Cells/cytology/ physiologyMegakaryocytes/cytology/physiologyPlatelet Glycoprotein GPIIb-IIIa Complex/biosynthesis/ geneticsRecombinant Proteins/pharmacologyStem Cell Factor/pharmacologyT-Lymphocytes/cytology/physiology
Stable URL
Full text
PMID: 10216084
140 hits and 0 download since 2010-08-27
Export document
Format :
Citation style :