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Effect of long-term anti-CD4 or anti-CD8 treatment on the development of lpr CD4- CD8- double negative T cells and of the autoimmune syndrome in MRL-lpr/lpr mice

Merino, Ramon
Fossati-Jimack, Liliane
Iwamoto, Masahiro
Takahashi, Satoru
Pugliatti, L.
Merino, Jesus
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Published in Journal of Autoimmunity. 1995, vol. 8, no. 1, p. 33-45
Abstract We have determined the effect of anti-CD4 or anti-CD8 monoclonal antibody (mAb) treatment from birth on the generation of the lpr CD4- CD8- double-negative (DN) T cell subset and on the development of lupus-like autoimmune syndrome in MRL-lpr/lpr mice. Both anti-CD4 and anti-CD8 mAb treatments resulted in a marked inhibition of lymph-adenopathy, whereas the development of the lpr DN T cells and of the lupus-like autoimmune syndrome strikingly differed in these two groups of mice. The treatment with anti-CD8 mAb almost completely blocked the appearance of the lpr DN T cells without any significant effect on the development of lupus-like autoimmune syndrome in MRL-lpr/lpr mice. In contrast, mice treated with anti-CD4 mAb failed to develop a lupus-like syndrome, while they still developed the lpr DN T cell subset, the predominant population in their lymph nodes, although absolute numbers were markedly diminished. Our results support the idea that CD8+ T cells are a major source of the lpr DN T cells, and that the lpr DN T cells play a minor, if any, role in the pathogenesis of lupus-like autoimmune syndrome in MRL-lpr/lpr mice.
Keywords AnimalsAntibodies, MonoclonalAntigens, CD4/ immunologyAntigens, CD8/ immunologyAutoimmune Diseases/ immunology/pathologyBlotting, NorthernCell Differentiation/immunologyFlow CytometryLupus Erythematosus, Systemic/immunologyLymph Nodes/pathologyMiceMice, Mutant StrainsT-Lymphocyte Subsets/ immunology
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PMID: 7734035
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