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Interferon-beta induces brain-derived neurotrophic factor in peripheral blood mononuclear cells of multiple sclerosis patients

Kantengwa, S.
Juillard, C.
Published in Journal of Neuroimmunology. 2008, vol. 197, no. 2, p. 147-51
Abstract Interferon-beta (IFN-beta) achieves its beneficial effect on multiple sclerosis (MS) via anti-inflammatory properties. In this study, we assessed the expression of the brain-derived neurotrophic factor (BDNF) in peripheral blood mononuclear cells (PBMC) from relapsing-remitting multiple sclerosis (RRMS) patients treated or not with IFN-beta. Intracellular BDNF was measured by Western blot and ELISA and compared with serum BDNF. We found higher levels of BDNF in PBMC of IFN-beta-treated versus non-treated patients, whereas serum levels of BDNF were similar. We hypothesize that the increased intracellular BDNF secondary to IFN-beta is not released in the periphery. This release is probably not tissue specific but in MS patients, BDNF could be specifically delivered by PBMC at the site of re-activation, i.e. within the central nervous system.
Keywords AdultBlotting, Western/methodsBrain-Derived Neurotrophic Factor/metabolismCase-Control StudiesDose-Response Relationship, DrugEnzyme-Linked Immunosorbent Assay/methodsFemaleGene Expression Regulation/drug effectsHumansImmunologic Factors/pharmacologyInterferon-beta/pharmacologyLeukocytes, Mononuclear/drug effectsMaleMiddle AgedMultiple Sclerosis/pathology
Stable URL http://archive-ouverte.unige.ch/unige:1142
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PMID: 18555540
Research group Scléroses en plaques, neuroimmunologie, encéphalite autoimmune expérimentale (843)
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