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Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

Miescher, P. A.
Published in Clinical and Experimental Immunology. 1976, vol. 26, no. 3, p. 425-430
Abstract A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.
Keywords Antibodies/ analysisBinding Sites, AntibodyDNA/ immunologyHumansImmunologic TechniquesLeukemia/immunologyLupus Erythematosus, Systemic/immunologyProtein Binding/drug effectsSodium Dodecyl Sulfate/pharmacology
Stable URL http://archive-ouverte.unige.ch/unige:11338
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PMID: 795575
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