UNIGE document Scientific Article
previous document  unige:11237  next document
add to browser collection

Actin isoforms in amphioxus Branchiostoma lanceolatum

Fagotti, A.
Di Rosa, I.
Simoncelli, F.
Pascolini, R.
Published in Cell and Tissue Research. 1998, vol. 292, no. 1, p. 173-176
Abstract Actin is a highly conserved cytoskeletal protein that is ubiquitous in all eukaryotes. Little is known about actin expression in amphioxus, the closest living relative of the vertebrates. In the present study, involving Western blotting and indirect immunofluorescence, we report the characterization and localization of various actin isoforms in amphioxus (Branchiostoma lanceolatum) tissues. Three antibodies against vertebrate actins were used: a polyclonal antibody recognizing beta-cytoplasmic actin (anti-beta actin), a monoclonal antibody against sarcomeric actins (anti-alphaSR-1), and a monoclonal antibody specific for alpha-smooth actin (anti-alphaSM-1). Western blot analysis of amphioxus extracts immunodecorated with these antibodies showed a 43-kDa-positive band co-migrating with respective controls. The amphioxus isoactin expression patterns recognized by these antibodies were similar to those of vertebrates, i.e., anti-beta actin showed positive staining mainly in non-muscle cells, anti-alphaSR-1 labelled dorsolateral myotomal muscles, and anti-alphaSM-1 stained ventral muscles. These results demonstrate that at least two muscle actins are present in amphioxus, suggesting that muscle actin gene duplication events began before vertebrate divergence from the amphioxus lineage.
Keywords Actins/ analysis/biosynthesisAnimalsBlotting, WesternChordata, Nonvertebrate/ chemistryFluorescent Antibody Technique, IndirectIsomerism
Stable URL http://archive-ouverte.unige.ch/unige:11237
Full text
PMID: 9506925
102 hits and 0 download since 2010-08-27
Export document
Format :
Citation style :