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c-fos gene transcription in murine macrophages is modulated by a calcium-dependent block to elongation in intron 1

Tourkine, N.
Jeanteur, P.
Blanchard, J. M.
Published in Molecular and Cellular Biology. 1991, vol. 11, no. 5, p. 2826-2831
Abstract Cultured mouse thioglycolate-elicited peritoneal macrophages exhibit a strong block to transcriptional elongation beyond the end of the c-fos gene first exon. This block is absent in freshly isolated peritoneal cells, appears slowly during culture, and does not require adherence of the cells. The extent of this block is largely responsible for the levels of c-fos mRNA in cultured macrophages, even after modulation by agents such as the tumor promoter phorbol myristate acetate and increased intracellular cyclic AMP, which also increase the activity of the c-fos promoter. When macrophages are cultured in the absence of mobilizable calcium, the block can no longer be relieved by any inducing agent. Conversely, upon calcium influxes, there is little alteration in the level of transcriptional initiation, but transcription proceeds efficiently through the entire c-fos locus. These results suggest the presence of an intragenic calcium-responsive element in the c-fos gene and illustrate its key role in the control of c-fos gene transcription.
Keywords AnimalsCalcimycin/pharmacologyCalcium/ pharmacology/physiologyCell Nucleus/physiologyCells, CulturedGene Expression Regulation/drug effectsIntronsMacrophages/drug effects/ physiologyMiceMice, Inbred CBAProtein-Tyrosine Kinases/geneticsProto-Oncogene Proteins/ geneticsProto-Oncogene Proteins c-fosProto-Oncogenes/drug effectsRNA, Messenger/geneticsTetradecanoylphorbol Acetate/pharmacologyTranscription, Genetic/ drug effects
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Other version: http://mcb.asm.org/cgi/reprint/11/5/2826.pdf
PMID: 1901949
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