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MicroRNA-155 modulates the interleukin-1 signaling pathway in activated human monocyte-derived dendritic cells

Ceppi, Maurizio
Pereira, P. M.
Santos, M. A.
Published in Proceedings of the National Academy of Sciences. 2009, vol. 106, no. 8, p. 2735-2740
Abstract In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms to control immunity. Here, we show that in response to Lipopolysaccharides (LPS), several microRNAs (miRNAs) are regulated in human monocyte-derived dendritic cells. Among these miRNAs, miR-155 is highly up-regulated during maturation. Using LNA silencing combined to microarray technology, we have identified the Toll-like receptor/interleukin-1 (TLR/IL-1) inflammatory pathway as a general target of miR-155. We further demonstrate that miR-155 directly controls the level of TAB2, an important signal transduction molecule. Our observations suggest, therefore, that in mature human DCs, miR-155 is part of a negative feedback loop, which down-modulates inflammatory cytokine production in response to microbial stimuli.
Keywords Adaptor Proteins, Signal Transducing/genetics/metabolismBase SequenceCells, CulturedDNA PrimersDendritic Cells/ metabolismHumansInterleukin-1/ metabolismLymphocyte ActivationMicroRNAs/ physiologyMonocytes/ metabolismRNA Processing, Post-TranscriptionalSignal Transduction/ physiologyToll-Like Receptors/metabolism
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PMID: 19193853
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