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Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice

Budd, R. C.
MacDonald, H. R.
Lowenthal, J. W.
Davignon, J. L.
Cerottini, J. C.
Published in Journal of Immunology. 1985, vol. 135, no. 6, p. 3704-3711
Abstract Mice bearing the recessive gene lpr develop an autoimmune syndrome associated with a massive lymphadenopathy, both of which are age and thymus dependent. The predominant accumulating cells in lymphoid tissue of lpr/lpr mice are Thy-1+ but express neither of the mature T cell markers, Lyt-2 or L3T4. We have purified this Lyt-2-/L3T4- subset and examined its phenotype. These cells are not actively cycling, do not express interleukin-2 (IL 2) receptors nor significant levels of antigen receptor, but do express the B cell marker B220. In vitro growth conditions were examined for the lpr Lyt-2-/L3T4- subset. By using a combination of phorbol ester and IL 2, these cells acquired transient expression of IL 2 receptors and grew in an IL 2-dependent manner. Furthermore, these proliferating cells underwent differentiation to a more mature T cell phenotype, with loss of cell surface B220 and acquisition, by a portion, of antigen receptor and Lyt-2. The possible parallels with normal T cell maturation are discussed.
Keywords AnimalsAntigens, Ly/geneticsAntigens, Surface/geneticsCell Cycle/drug effectsCell Differentiation/drug effectsConcanavalin A/pharmacologyLymph Nodes/cytologyLymphocyte Activation/drug effectsLymphocyte Function-Associated Antigen-1MiceMice, Inbred C57BL/ geneticsPhenotypeReceptors, Immunologic/metabolismReceptors, Interleukin-2T-Lymphocytes, Cytotoxic/ classification/cytology/immunology/metabolismTetradecanoylphorbol Acetate/pharmacology
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PMID: 3934265
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