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Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4

Hedgpeth, J.
Selzer, G. B.
Epstein, R. H.
Published in Proceedings of the National Academy of Sciences. 1979, vol. 76, no. 2, p. 700-704
Abstract We have determined the sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo. The rIIB mutant HD263, which is temperature sensitive for translation, has an altered initiation codon (AUA instead of AUG). Because wild-type and HD263 proteins have the same fMet peptide, this AUA is used to initiate translation in the mutant rIIB mRNA. We have also identified a six-base sequence which is complementary to the 3" end of the 16S rRNA.
Keywords Base SequenceBinding SitesCodonColiphages/ geneticsGenes, ViralMutationPeptide Chain Initiation, TranslationalRNA, Messenger/geneticsRibosomes/metabolismTemperature
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PMID: 284393
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