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Genetic engineering of embryonic stem cells

Suter, D. M.
Published in Schweizerische medizinische Wochenschrift. 2006, vol. 136, no. 27-28, p. 413-415
Abstract We describe a novel generation of lentiviral vectors that are particularly well suited for work with embryonic stem cells. The possibility of selecting cell lines with antibiotics and the rapid insertion of any combination of promoters and genes of interest makes them a powerful tool in the generation of transgenic ES cell lines. This vector can also greatly facilitate studies aimed at the improvement of neuronal engineering from ES cells, by making it possible to monitor the emergence and differentiation of neurons.
Keywords AnimalsCell Differentiation/geneticsEmbryo, Mammalian/ cytologyGene ExpressionGenetic EngineeringGenetic Vectors/geneticsHIV/geneticsHumansNeurons/transplantationStem Cell TransplantationStem Cells/physiology
Stable URL http://archive-ouverte.unige.ch/unige:11061
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Other version: http://www.smw.ch/docs/pdf200x/2006/27/smw-11406.pdf
PMID: 16897894
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