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The determination of huperzine A in european Lycopodiaceae species by HPLC-UV-MS

Borloz, A.
Marston, Andrew
Published in Phytochemical Analysis. 2006, vol. 17, no. 5, p. 332 - 336
Abstract A rapid qualitative HPLC-UV-MS method was developed for the detection of huperzine A in Lycopodiaceac species. HPLC coupled with mass spectrometry experiments allowed the identification of the alkaloid and enabled targeted analysis of complex matrices such as herbal extracts. Huperzine A was detected by single ion monitoring of the pseudomolecular ion [M + H](+) at m/z 243.2. The alkaloid was also detected on TLC in a bioautographic enzyme assay with acetylcholinesterase to show the activity of the compound. Four Lycopodiaceae species collected in Switzerland were tested by these methods and Huperzia selago (L.) Bernh. ex Schrank et Martius was the only species found to contain huperzine A. Copyright (c) 2006 John Wiley & Sons, Ltd.
Keywords HPLC-UV-MSTLCHuperzine ALycopodiaceaeHuperzia selago
Stable URL http://archive-ouverte.unige.ch/unige:11022
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Other version: http://doi.wiley.com/10.1002/pca.922
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