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Expression and function of junctional adhesion molecule-C in human and experimental arthritis

Palmer, Gaby
Busso, Nathalie
Chobaz-Peclat, Veronique
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Published in Arthritis Research and Therapy. 2007, vol. 9, no. 4, p. R65
Abstract Junctional adhesion molecule-C (JAM-C) is an adhesion molecule involved in transendothelial migration of leukocytes. In this study, we examined JAM-C expression in the synovium and investigated the role of this molecule in two experimental mouse models of arthritis. JAM-C expression was investigated by reverse transcriptase-polymerase chain reaction and immunohistochemistry. The effects of a monoclonal anti-JAM-C antibody were assessed in antigen-induced arthritis (AIA) and K/BxN serum transfer-induced arthritis. JAM-C was expressed by synovial fibroblasts in the lining layer and associated with vessels in the sublining layer in human and mouse arthritic synovial tissue. In human tissue, JAM-C expression was increased in rheumatoid arthritis (RA) as compared to osteoarthritis synovial samples (12.7 +/- 1.3 arbitrary units in RA versus 3.3 +/- 1.1 in OA; p < 0.05). Treatment of mice with a monoclonal anti-JAM-C antibody decreased the severity of AIA. Neutrophil infiltration into inflamed joints was selectively reduced as compared to T-lymphocyte and macrophage infiltration (0.8 +/- 0.3 arbitrary units in anti-JAM-C-treated versus 2.3 +/- 0.6 in isotype-matched control antibody-treated mice; p < 0.05). Circulating levels of the acute-phase protein serum amyloid A as well as antigen-specific and concanavalin A-induced spleen T-cell responses were significantly decreased in anti-JAM-C antibody-treated mice. In the serum transfer-induced arthritis model, treatment with the anti-JAM-C antibody delayed the onset of arthritis. JAM-C is highly expressed by synovial fibroblasts in RA. Treatment of mice with an anti-JAM-C antibody significantly reduced the severity of AIA and delayed the onset of serum transfer-induced arthritis, suggesting a role for JAM-C in the pathogenesis of arthritis.
Keywords Adoptive TransferAnimalsAntibodies, Blocking/pharmacologyArthritis, Experimental/drug therapy/ metabolism/pathologyArthritis, Rheumatoid/ metabolism/pathologyCell Adhesion Molecules/genetics/immunology/ metabolismCells, CulturedDisease Models, AnimalFibroblasts/drug effects/metabolism/pathologyGene ExpressionHumansImmunoglobulins/genetics/immunology/ metabolismMacrophagesMaleMembrane Proteins/genetics/immunology/ metabolismMiceMice, Inbred C57BLMice, TransgenicNeutrophilsOsteoarthritis/ metabolism/pathologyRNA, Messenger/metabolismSerum Amyloid A Protein/analysisSpleen/drug effects/pathologySynovial Membrane/chemistry/ metabolismT-Lymphocytes/drug effects/pathology
Stable URL http://archive-ouverte.unige.ch/unige:11002
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PMID: 17612407
Research group Mécanisme de l'inflammation articulaire (44)
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