Monoclonal antibodies NORM-1 and NORM-2 induce more normal behavior of tumor cells in vitro and reduce tumor growth in vivo
|Published in||Cell. 1985, vol. 40, no. 3, p. 547-557|
|Abstract||In this study, large numbers of hybridomas (produced by syngeneic immunization with B16 mouse melanoma and fusion with NS-1 myeloma cells) were screened for the production of antibodies that affected morphology and growth of animal and human tumor cells in vitro. Two such antibodies, NORM-1 and NORM-2 (both IgG2a), inhibited the growth of B16 melanoma cells in soft agar and increased the serum requirements of tumor cells in tissue culture. Antibody NORM-2 also inhibited the growth of SV40-transformed 3T3 cells in agar and caused them to deposit more fibronectin into extracellular matrix. These antibodies thus seem to induce a more normal behavior of tumor cells in vitro. In vivo both antibodies reduced the number of growing lung tumors of B16 melanoma in C57BL/6 mice by 70%-90% when injected 3 days after the tumor cells. By immunoprecipitation of 35S-methionine-labeled cell extracts, NORM-2 antibody recognized a 59 kd protein in B16 mouse and in A375 human melanoma cells but not in 3T3 fibroblasts.|
|Keywords||Animals — Antibodies, Monoclonal/ physiology — Carcinoma/pathology — Cell Adhesion — Cell Line — Cell Transformation, Neoplastic — Cell Transformation, Viral — Fibronectins/metabolism — Humans — Hybridomas/immunology — Immunosorbent Techniques — Lung Neoplasms/secondary — Melanoma/pathology — Mice — Mice, Inbred C57BL — Neoplasms/ pathology — Simian virus 40 — Teratoma/pathology|
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