Gelsolin-actin complex is target for ADP-ribosylation by Clostridium botulinum C2 toxin in intact human neutrophils
|Published in||European Journal of Pharmacology. 1993, vol. 246, no. 3, p. 293-297|
|Abstract||The gelsolin-actin complex was ADP-ribosylated by Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin in lysates of human platelets and human neutrophils. When [32P]orthophosphate-labelled human neutrophils were treated with C. botulinum C2 toxin, [32P]ADP-ribosylated gelsolin-actin was precipitated with anti-gelsolin antibody. Stimulation of neutrophils by formyl-methionine-leucine-phenylalanine decreased the interaction of gelsolin with ADP-ribosylated actin in toxin-treated cells. The data indicate that the gelsolin-actin complex is a pathophysiological substrate for actin-ADP-ribosylating toxins.|
|Keywords||Actins/chemistry/ metabolism — Adenosine Diphosphate Ribose/ metabolism — Blood Platelets/drug effects/metabolism — Botulinum Toxins/ pharmacology — Gelsolin/chemistry/ metabolism — Humans — Neutrophils/ drug effects/metabolism|
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