UNIGE document Scientific Article
previous document  unige:10918  next document
add to browser collection
Title

IgG rheumatoid factors and cryoglobulins in mice bearing the mutant gene lpr (lymphoproliferation)

Authors
Abdelmoula, M.
Gyotoku, Y.
Lange, G.
Published in Rheumatology International. 1984, vol. 4 Suppl, p. 45-48
Abstract The autosomal recessive mutant gene, lpr (lymphoproliferation), produces a massive proliferation of T cells in autoimmune MRL mice. Since the MRL strain bearing the lpr mutation is the only strain that spontaneously develops high titers of rheumatoid factors (RF), we have investigated whether non-autoimmune mice (C57BL/6, C3H/HeJ and AKR) bearing the lpr gene could produce RF. Serum levels of RF activity were assessed by a new radioimmunoassay, in which serum samples pretreated with acetate buffer were incubated with 125I-mouse IgG and precipitated with 7% polyethylene glycol. The majority of serum from 4- to 6-month-old non-autoimmune strains of mice bearing the lpr gene exhibited significant RF activity, as did MRL-lpr/lpr mice. Sucrose density-gradient analysis has revealed that all the IgG RF activity was present in a form of immune complexes, sedimenting in the intermediate position (7-19S) and fully dissociable into 7S IgG under an acid condition. This indicates that the production of IgG RF does not require a particular background genome of the MRL strain. In addition, mice bearing the lpr mutation developed extremely large amounts of cryoglobulins, which paralleled the production of RF. Analysis of components of their cryoglobulins revealed a marked enrichment of IgG3 subclass as compared to other IgG subclasses and IgM. These results suggest that IgG3-containing immune complexes represent the major source of cryoglobulins occurring in mice bearing the lpr gene.
Keywords AnimalsAntigen-Antibody Complex/geneticsAutoimmune Diseases/genetics/immunologyCryoglobulins/ geneticsFemaleGenes, RecessiveImmunoglobulin G/ geneticsLymphoproliferative Disorders/ genetics/immunologyMaleMiceMice, Mutant StrainsMutationRheumatoid Factor/ geneticsT-Lymphocytes/immunology
Stable URL http://archive-ouverte.unige.ch/unige:10918
Full text
Identifiers
PMID: 6336225
Structures
131 hits and 0 download since 2010-08-26
Update
Export document
Format :
Citation style :