UNIGE document Scientific Article
previous document  unige:10906  next document
add to browser collection

Fate of undifferentiated mouse embryonic stem cells within the rat heart: role of myocardial infarction and immune suppression

Trindade, P. T.
Stumm, Michael
Zammaretti, Prisca
Bettiol, Esther
show hidden authors show all authors [1 - 11]
Published in Journal of Cellular and Molecular Medicine. 2009, vol. 13, no. 1, p. 188-201
Abstract It has recently been suggested that the infarcted rat heart microenvironment could direct pluripotent mouse embryonic stem cells to differentiate into cardiomyocytes through an in situ paracrine action. To investigate whether the heart can function as a cardiogenic niche and confer an immune privilege to embryonic stem cells, we assessed the cardiac differentiation potential of undifferentiated mouse embryonic stem cells (mESC) injected into normal, acutely or chronically infarcted rat hearts. We found that mESC survival depended on immunosuppression both in normal and infarcted hearts. However, upon Cyclosporin A treatment, both normal and infarcted rat hearts failed to induce selective cardiac differentiation of implanted mESC. Instead, teratomas developed in normal and infarcted rat hearts 1 week and 4 weeks (50% and 100%, respectively) after cell injection. Tight control of ESC commitment into a specific cardiac lineage is mandatory to avoid the risk of uncontrolled growth and tumourigenesis following transplantation of highly plastic cells into a diseased myocardium.
Keywords AnimalsCell Differentiation/ physiologyCell LineageCyclosporine/metabolismEmbryonic Stem Cells/cytology/ physiology/ transplantationHumansImmunosuppressionImmunosuppressive Agents/metabolismMaleMiceMyocardial Infarction/ pathologyMyocardium/cytology/pathologyProto-Oncogene Proteins c-bcl-2/genetics/metabolismRatsRats, Sprague-DawleyTeratoma/metabolism/pathologyTransplantation, HeterologousVentricular Function, Left
Stable URL http://archive-ouverte.unige.ch/unige:10906
Full text
PMID: 18373734
Research groups Mécanismes de différentiation cellulaire des cellules cardiaques (536)
Radicaux libres et cellules souches embryonnaires (60)
139 hits and 0 download since 2010-08-26
Export document
Format :
Citation style :