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Title

Actin organization and fibrin-clot retractile activity of cultured mouse fibroblasts

Authors
Curatolo, L.
Donati, M. B.
Morasca, L.
Published in Cell and Tissue Research. 1982, vol. 223, no. 3, p. 665-673
Abstract It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a powerful actin-depolymerizing factor (ADF), present in plasma and serum of humans and laboratory animals was investigated. Fibroblasts at early passages (2-4) were tested for these parameters at various intervals after seeding (24, 96, and 168 h). The number of actin stress-lines was progressively higher, while the sensitivity to ADF action was progressively lower in cells cultured from 24 to 168 h; the FCR capacity was significantly decreased at 168 h. These data suggest that cells containing weakly polymerized and/or stabilized actin are more active than those containing highly polymerized and/or stabilized actin in triggering fibroblast contraction.
Keywords Actin Depolymerizing FactorsActins/ physiologyAnimalsBlood Proteins/physiologyCells, CulturedDestrinFemaleFibrin/ physiologyFibroblasts/ultrastructureMiceMicrofilament ProteinsPregnancy
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PMID: 7094000
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