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Suppression of B cell development and antibody responses in mice with polyclonal rabbit and monoclonal rat anti-IgM antibodies. I. Characterization of the suppressed state

Cerny, A.
Hugin, A. W.
Sutter, S.
Heusser, C. H.
Bos, N.
Hengartner, Hans
Zinkernagel, R. M.
Published in Experimental Cell Biology. 1985, vol. 53, no. 6, p. 301-313
Abstract Mice treated from birth with polyclonal, crude or affinity purified rabbit or monoclonal rat anti-mouse IgM antibodies [b-7-6 and C-2-23: Eur. J. Immunol. 14: 753-757, 1984] were found to be heavily suppressed with respect to B-cell activities. Crude or affinity purified rabbit or monoclonal rat anti-mouse IgM gave comparable results as follows: serum IgM was below detectable levels; serum IgG was reduced to about 1-3% of normal levels; free anti-IgM was always detectable; IgM and/or kappa-light-chain positive cells as well as IgM-secreting cells were absent in various lymphoid organs; the B-cell mitogen lipopolysaccharide was unable to induce proliferative responses; primary antibody responses could not be induced against sheep red blood cells and phosphorylcholine; lymphoid organs were reduced in size and B-cell areas were not populated with lymphocytes; besides a 40% reduction in absolute lymphocyte numbers in the blood, we found increased platelet counts and a 10% eosinophilia in anti-IgM-treated mice.
Keywords AnimalsAntibodies, Anti-Idiotypic/ administration & dosage/ physiologyAntibodies, Monoclonal/ administration & dosageAntibody FormationB-Lymphocytes/ immunologyBone Marrow CellsHemolytic Plaque TechniqueImmune Sera/administration & dosageImmune ToleranceImmunoglobulin G/biosynthesis/metabolismImmunoglobulin M/biosynthesis/immunology/ physiologyLymph Nodes/cytologyLymphocyte ActivationMiceMice, Inbred BALB CMice, Inbred C57BLRabbitsRatsSpleen/cytology
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PMID: 3908182
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