UNIGE document Scientific Article
previous document  unige:10845  next document
add to browser collection
Title

The role of E-selectin for neutrophil activation and tumor metastasis in vivo

Authors
Araki, Masatake
Araki, Kimi
Biancone, L.
Stamenkovic, I.
Yamamura, K.
Published in Leukemia. 1997, vol. 11, no. Suppl 3, p. 209-212
Abstract The selectin class of adhesion molecules plays a critical role in facilitating leukocyte adhesion to and subsequent transmigration of endothelium. On this basis, selectins have been suggested to promote metastasis of certain types of tumors, although direct evidence is lacking. To explore this hypothesis two sets of transgenic mice were developed, one TgNES, which constitutively expresses cell surface E-selectin in all tissues, the other TgNEsol, which expresses truncated, soluble E-selectin in the liver. Both transgenic mice showed apparently normal phenotype. However in TgNES mice, but not in TgNEsol mice, the number of neutrophil decreased to 50% compared with that in their negative littermate. And also these neutrophils were markedly activated. On the other hand, B16 F10 melanoma cells were stably transfected with alpha 1-3/4 fucosyltransferase-specific cDNA (B16F10 ft), allowing them to express E-selectin ligands. Normal mice injected with B16F10 ft developed lung tumors exclusively. In contrast, TgNES mice developed massive, infiltrating liver tumors. TgNEsol mice also developed liver tumors that grow more slowly. These observations suggest the important role of E-selectin for neutrophil activation and tumor metastasis in vivo.
Keywords Actins/biosynthesis/geneticsAnimalsChickensE-Selectin/biosynthesis/genetics/ physiologyFucosyltransferases/biosynthesis/geneticsLiver/metabolismMelanoma, Experimental/ immunology/pathology/ physiopathologyMiceMice, Inbred C57BLMice, TransgenicNeoplasm MetastasisNeutrophil ActivationNeutrophils/ physiologyRecombinant Proteins/biosynthesisTransfection
Stable URL http://archive-ouverte.unige.ch/unige:10845
Full text
This document has no fulltext available yet, but you can contact its author by using the form below.
Identifiers
PMID: 9209343
Structures
105 hits and 0 download since 2010-08-26
Update
Export document
Format :
Citation style :