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Title

Lactate-induced retinal arteriolar vasodilation implicates neuronal nitric oxide synthesis in minipigs

Authors
Stangos, Alexandros N.
Published in Investigative Ophthalmology and Visual Science, IOVS. 2008, vol. 49, no. 11, p. 5060-6
Abstract PURPOSE: To investigate the role of neuronal nitric oxide (NO) synthesis in the retinal vasodilatory response to lactate in minipigs. METHODS: Thirteen eyes of 13 minipigs were evaluated. Ten eyes received an intravenous infusion of N(omega)-nitro-L-arginine methyl ester (L-NAME). After 1 hour, the same eyes received an intravitreous juxta-arteriolar microinjection of 30 microL of L-lactate 0.5 M (pH 7.4) through a micropipette. Ten minutes later, 9 of 10 eyes received an intravitreous juxta-arteriolar microinjection of 30 microL of L-NAME 0.01 M (pH 7.4), and 1 received physiologic saline solution (PSS). The remaining three eyes received a microinjection of 30 microL of L-lactate 0.5 M (pH 7.4), without intravenous or intravitreous L-NAME. RESULTS: The three eyes that received juxta-arteriolar injection of L-lactate only showed a reproducible increase in retinal arteriolar diameter that persisted during the entire study period (maximum effect at 20 minutes, 40.9% +/- 3.2%). Retinal arteriolar diameter decreased by 4.1% 1 hour after intravenous L-NAME when compared with baseline but the difference did not reach significance. The juxta-arteriolar injection of L-lactate induced a significant increase in retinal arteriolar diameter (22.7% and 28.7% at 5 and 10 minutes, respectively; P < 0.01), followed by a significant decrease (8.6%; P < 0.01) 10 minutes after juxta-arteriolar injection of L-NAME. Injection of PSS had no effect on retinal arteriolar diameter. CONCLUSIONS: Juxta-arteriolar administration of L-lactate induced vasodilation, which was also observed with continuous intravenous infusion of L-NAME. Moreover, juxta-arteriolar L-NAME microinjection significantly suppressed the vasodilatory effect of L-lactate. These data suggest that neuronal-derived NO is an important mediator of lactate-induced vasodilation in minipigs.
Keywords AnimalsArterioles/cytology/drug effects/physiologyBlood Pressure/physiologyDose-Response Relationship, DrugEndothelium, Vascular/cytology/metabolismEnzyme Inhibitors/administration & dosageInfusions, IntravenousMicroinjectionsNG-Nitroarginine Methyl Ester/administration & dosageNitric Oxide/biosynthesisRetinal Artery/cytology/drug effects/physiologySwineSwine, MiniatureVasodilation/drug effectsVitreous Body
Stable URL http://archive-ouverte.unige.ch/unige:1078
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Other version: http://www.iovs.org/cgi/content/full/49/11/5060
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PMID: 18599561
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