UNIGE document Scientific Article
previous document  unige:10529  next document
add to browser collection
Title

The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes

Authors
Zuber, Benoit
Nikonenko, Irina
Dubochet, Jacques
Published in Proceedings of the National Academy of Sciences. 2005, vol. 102, no. 52, p. 19192-19197
Abstract Cryo-electron microscopy of vitreous section makes it possible to observe cells and tissues at high resolution in a close-to-native state. The specimen remains hydrated; chemical fixation and staining are fully avoided. There is minimal molecular aggregation and the density observed in the image corresponds to the density in the object. Accordingly, organotypic hippocampal rat slices were vitrified under high pressure and controlled cryoprotection conditions, cryosectioned at a final thickness of approximately 70 nm and observed below -170 degrees C in a transmission electron microscope. The general aspect of the tissue compares with previous electron microscopy observations. The detailed analysis of the synapse reveals that the density of material in the synaptic cleft is high, even higher than in the cytoplasm, and that it is organized in 8.2-nm periodic transcleft complexes. Previously undescribed structures of presynaptic and postsynaptic elements are also described.
Keywords AnimalsBiopsyCentral Nervous System/ pathologyCryoelectron MicroscopyElectrophysiologyFreezingHippocampus/ pathologyMicroscopy, ElectronMicroscopy, Electron, TransmissionNerve TissueNervous System/pathologyPeripheral Nervous System/pathologyPressureRatsSynapsesTemperatureTime Factors
Stable URL http://archive-ouverte.unige.ch/unige:10529
Full text
Identifiers
PMID: 16354833
Structures
115 hits and 0 download since 2010-08-06
Update
Export document
Format :
Citation style :