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Title

A new interface chamber for the study of mammalian nervous tissue slices

Authors
Reynaud, J. C.
Martini, F.
Chatel, C.
Buclin, M.
Puizillout, J. J.
Published in Journal of Neuroscience Methods. 1995, vol. 58, no. 1-2, p. 203-208
Abstract We describe a new interface-type chamber for electrophysiological studies in mammalian brain slices. Thermoregulation of the inner recording chamber is achieved using the Peltier effect and a feedback control unit. Between 15 and 40 degrees C, and for perfusion rates from 1 to 5 ml/min, the temperature can be maintained within +/- 0.1 degrees C of the command value; it can also be rapidly and reliably changed. An external bath, heated by a coiled resistor, generates a humidified, oxygenated atmosphere diffusing above the slices. Survival of neuronal tissue is excellent and stable intracellular recordings can be obtained using either sharp or patch-clamp micropipettes. Perfusion solutions can be readily exchanged, rendering this chamber suitable for the study of bath-applied neuroactive compounds.
Keywords AnimalsBrain/physiologyDiffusion Chambers, CultureElectrophysiology/ instrumentationNerve Tissue/ physiologyNeurons/physiologyPatch-Clamp TechniquesPerfusion/instrumentationRatsTemperatureVagus Nerve/physiology
Stable URL http://archive-ouverte.unige.ch/unige:10423
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PMID: 7475228
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