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Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion
|Published in||FEBS Letters. 1998, vol. 434, no. 1-2, p. 177-182|
|Abstract||An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K+ current, I(K(NI)), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K+ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32-41, and is expressed as a approximately 9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I(K(NI)) indicating that h-eag constitutes the channel responsible for this current in vivo.|
|Keywords||Adolescent — Adult — Amino Acid Sequence — Brain/metabolism — Cell Differentiation — Cells, Cultured — Child — Child, Preschool — Cloning, Molecular — Ether-A-Go-Go Potassium Channels — Humans — Infant — Molecular Sequence Data — Muscle, Skeletal/cytology/ metabolism — Potassium Channels/biosynthesis/ genetics — Sequence Alignment — Sequence Analysis|