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Title

T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts

Authors
Bijlenga, P.
Liu, J. H.
Espinos, E.
Published in Proceedings of the National Academy of Sciences. 2000, vol. 97, no. 13, p. 7627-7632
Abstract Mechanisms underlying Ca(2+) signaling during human myoblast terminal differentiation were studied using cell cultures. We found that T-type Ca(2+) channels (T-channels) are expressed in myoblasts just before fusion. Their inhibition by amiloride or Ni(2+) suppresses fusion and prevents an intracellular Ca(2+) concentration increase normally observed at the onset of fusion. The use of antisense oligonucleotides indicates that the functional T-channels are formed by alpha1H subunits. At hyperpolarized potentials, these channels allow a window current sufficient to increase [Ca(2+)](i). As hyperpolarization is a prerequisite to myoblast fusion, we conclude that the Ca(2+) signal required for fusion is produced when the resting potential enters the T-channel window. A similar mechanism could operate in other cell types of which differentiation implicates membrane hyperpolarization.
Keywords Calcium/ metabolismCalcium Channels, T-Type/ metabolismCell DifferentiationCells, CulturedHumansIon TransportMuscle, Skeletal/cytology/ metabolismSignal Transduction
Stable URL http://archive-ouverte.unige.ch/unige:10191
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PMID: 10861024
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