UNIGE document Scientific Article
previous document  unige:10098  next document
add to browser collection
Title

The isolated and perfused brain of the guinea-pig in vitro

Authors
de Curtis, M.
Walton, K.
Llinas, R.
Published in European Journal of Neuroscience. 1993, vol. 5, no. 7, p. 915-926
Abstract We describe here an isolated and perfused in vitro adult guinea-pig whole brain preparation which is an extension of the previously described in vitro brainstem-cerebellum preparation. Viability was tested by the analysis of trans-synaptic responses along the visual pathways following the electrical stimulation of the optic nerve or the optic radiations. The evoked field potentials were recorded in the dorsal lateral geniculate, the superior colliculus and the visual cortex. The distribution of extracellular currents was studied using current source density analysis, in order to determine the amplitude, time course and spatial organization of the synaptic activity at these sites. The study indicates that field potentials were very similar to those described in vivo. These data demonstrate the survival of a complex adult sensory system in vitro and suggest that this preparation can be used for the analysis of multisynaptic circuits in the mammalian brain.
Keywords AnimalsBrain/ physiologyElectric StimulationElectrophysiologyEvoked PotentialsGeniculate Bodies/physiologyGuinea PigsPerfusionSuperior Colliculi/physiologyVisual Cortex/physiologyVisual Pathways/physiology
Stable URL http://archive-ouverte.unige.ch/unige:10098
Full text
Identifiers
PMID: 8281302
Structures
144 hits and 0 download since 2010-08-06
Update
Export document
Format :
Citation style :