UNIGE document Scientific Article
previous document  unige:10085  next document
add to browser collection
Title

Human skeletal muscle has a voltage-gated proton current

Authors
Krause, R. M.
Published in Neuromuscular Disorders. 1993, vol. 3, no. 5-6, p. 407-411
Abstract A voltage-gated proton current, IH, was studied with the whole-cell patch-clamp technique in human myotubes obtained from biopsies of human muscle. Studies of the reversal potential of IH during substitution of K+, Na+, Ca2+, Cl-, Cs+, and H+ in the extracellular solution indicated that protons were the major charge carriers of IH. This current is similar in many respects, but not identical, to the proton currents already described in other cell types. IH is activated by depolarization and it can be affected by extracellular pH. IH can be blocked by external divalent cations including Ca2+. This block is voltage-dependent, being more efficient at hyperpolarized than at depolarized voltages. The voltage-dependent properties of IH and its ability to be affected by pH and extracellular Ca2+ suggest that IH might be used by muscle cells to extrude protons during action potentials.
Keywords Aspartic Acid/pharmacologyCalcium/pharmacologyCations, Divalent/pharmacologyCations, Monovalent/pharmacologyCells, CulturedChildEgtazic Acid/analogs & derivatives/pharmacologyHumansIon Channel Gating/drug effectsIon Channels/drug effects/ physiologyMagnesium Chloride/pharmacologyMeglumine/pharmacologyMembrane Potentials/drug effects/physiologyMuscles/ physiology
Stable URL http://archive-ouverte.unige.ch/unige:10085
Full text
This document has no fulltext available yet, but you can contact its author by using the form below.
Identifiers
PMID: 7514465
Structures
129 hits and 0 download since 2010-08-06
Update
Export document
Format :
Citation style :