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An improved method for Lowicryl K4M electron microscopic embedding of brain tissue

Published in Journal of Neuroscience Methods. 1989, vol. 30, no. 1, p. 29-32
Abstract The Lowicryl K4M low-temperature embedding technique was modified for brain tissue to facilitate antigen localization in neuronal structures by using postembedding immunoelectron microscopy. Rat brains fixed by perfusion with a mixture of 1% glutaraldehyde and 1% paraformaldehyde, were sectioned into 200-microns-thick coronal sections, and the appropriate area of the sections further microdissected into small samples (200 x 700 x 700 microns). These samples were processed for K4M embedding using prolonged incubation times at each step. This resulted in a homogeneously infiltrated tissue with good preservation of ultrastructure and antigen reactivity. Despite the lack of osmium fixation, the morphology of the synapses was clearly identifiable.
Keywords Acrylic ResinsAnimalsBrain/metabolism/ ultrastructureImmunohistochemistry/ methodsMaleMicroscopy, Electron/ methodsMicrotomy/ methodsNeurotransmitter Agents/ metabolismRats
Stable URL http://archive-ouverte.unige.ch/unige:10075
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PMID: 2572734
Research group Progéniteurs neuronaux
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